合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。     

舟山群岛一次"晴天暴"过程分析

主要使用常规填图资料计算各种物理量,对一次“晴天暴”大风过程进行诊断分析,结果表明此次大风的形成机制是:在强盛的西北急流作用下,急行性干冷锋快速东移南下,形成中低层的强温度梯度和地面气压梯度,高空冷平流与地面加热共同作用,形成大的温度层结递减率,产生不稳定层结,引起垂直动量交换,因此地面出现强风。最后总结出此类大风的预报思路。     

Numerical Simulation of Dynamic Response of Supple Nets

1 .Introduction Owing to the decline of capture fisheries andsaturation of traditional inshorefarms ,sea cage cul-ture as a newtype of aquaculture is developingrapidlyin China .However ,cage culture is a highriskoperation.The practice is vulnerable to natural hazards ,such as strong tides ,storms and typhoons .So,it is necessaryto assess the behavior of a sea cage exposedto waves and current before the designandinstallation of the structure . Computer simulation,due to its many advantages ,ha…     

Molecular phylogenetic analysis of sulfate-reducing bacteria from deep sediment layers of the tropical West Pacific warm pool

1IntroductionThe tropical West Pacific warm pool(TWP-WP),which spans an area roughly between10°Nto10°S of the equator from Indonesia to the dateline,has the world’s warmest sea surface temperature ofbeing greater than29℃.With the increase of recog-niz…     

基于GIS的柘林湾生态信息系统

柘林湾生态信息系统以ArcGIS为开发平台，采用客户端／服务器的体系结构，基于ArcObjects的组件式二次开发而成，实现了柘林湾的三维实景模拟、生态数学模型与GIS的有效集成、分析评价结果与地理空间数据相结合等功能，具有可视化、智能化、易扩展、安全实用等特点．该系统的建成．极大地提高了柘林湾生态信息的现代化管理水平，并为制定柘林湾的污染防治与生态环境保护策略提供了强有力的支持．     

南海季风型海-气耦合模态的时空特征及其与中国夏季降水的关系

使用NCEP/NCAR的海表温度(SST)、海面10 m风场的月平均再分析资料,用联合SVD(CSVD)的方法研究了不同季节南海的海气耦合模的时空分布特征及其与中国夏季降水的关系。通过对不同季节的海-气耦合模的年际变化特征的分析。结果发现:第一模态为最显著模态,模态协方差贡献比在四季均超过80%,空间上SST表现为与南海等深线相一致的海盆模态,风场上主要表现为弱的冬季风或弱的夏季风,各个季节的海-气耦合模态都主要反映了SST-蒸发-风反馈这样1种正反馈的海-气相互作用过程,而且冬季风期间这种相互作用要更强烈些。时间系数均主要表现为一致的上升趋势和1976年前后的年代际突变,以及与ENSO相关的年际变化特征。冬、夏季弱的季风对应暖SST的特征体现了这种耦合模态隔季相关的特征,都对应夏季华南旱(涝)、江南涝(旱),华北、山东半岛旱(涝),东北涝(旱)这样1种波列状的旱涝相间分布。     

PROBABILITY CHARACTERISTICS OF WAVE AND WAVE PRESSURES IN FRONT OF VERTICAL BREAKWATER

The distribution of wave height in front of a vertical breakwater, the range of wave pressure fluctuation at different subsurface levels, and the wave periods have shown that they are practically invariable with depth and can be determined theoretically. The distribution of crest and trough of total pressures is the same as that of wave height. As far as different subsurface levels are concerned, the spectral constitution of wave pressure field and the regularity of attenuation of spectral components are analyzed at the vertical breakwater, and a new expression describing the equilibrium range of wave pressure spectrum obtained.     

One new species of free-living marine nematodes (Enoplida,Anticomidae, Cephalanticoma) from the Huanghai Sea

One new species of free-living marine nematodes Cephalanticoma filicaudata sp. nov. from the Huanghai Sea, China is described and illustrated. Cephalanticoma filicaudata sp. nov. is characterized by well developed cephalic capsule, three teeth at the anterior of oesophagus, excretory pore opens posterior to cervical setae, only two cervical setae per file, body length longer than 5 200 μm, tail long and no ventral-lateral seta between the supplement and the cloacal opening. Types are deposited in the College of Marine Life Sciences, Ocean University of China.     

STWAVE模型在多岛屿区域波浪要素计算中的应用

我国常用的风推浪计算方法在多岛屿区域波浪要素计算中具有一定局限性。论文通过不同方法计算的波浪要素计算结果的比较,认为STWAVE模型在满足计算精度的前提下,操作简单,方法简便,在多岛屿区域波浪要素计算中具有较大的应用、推广价值。     

Phylogenetic analysis of Pectinidae(Bivalvia) based on the ribosomal DNA internal transcribed spacer region

The ribosomal DNA internal transcribed spacer (ITS) region is a useful genomic region for understanding evolutionary and genetic relationships. In the current study, the molecular phylogenetic analysis of Pectinidae (Mollusca: Bivalvia) was performed using the nucleotide sequences of the nuclear ITS region in nine species of this family. The sequences were obtained from the scallop species Argopecten irradians, Mizuhopecten yessoensis, Amusium pleuronectes and Mimachlamys nobilis, and compared with the published sequences of Aequipecten opercularis, Chlamys farreri, C. distorta, M. varia, Pecten maximus, and an outgroup species Perna viridis. The molecular phylogenetic tree was constructed by the neighbor-joining and maximum parsimony methods. Phylogenetic analysis based on ITS1, ITS2, or their combination always yielded trees of similar topology. The results support the morphological classifications of bivalve and are nearly consistent with classification of two subfamilies (Chlamydinae and Pectininae) formulated by Waller. However, A. irradians, together with A. opercularis made up of genera Amusium, evidences that they may belong to the subfamily Pectinidae. The data are incompatible with the conclusion of Waller who placed them in Chlamydinae by morphological characteristics. These results provide new insights into the evolutionary relationships among scallop species and contribute to the improvement of existing classification systems.